Further observations on the ultrastructure of ephemeral fever virus.
نویسنده
چکیده
Electron microscopic studies on ephemeral fever virus (EFV) have shown that the virus is cone-shaped having a basal diameter of approximately 176 m/k and a height of about 88 m/k (Lecatsas, Theodoridis & Erasmus, 1969). These workers have also shown that the virus has an envelope derived from the host cell membrane and that the internal electron-dense component consists of a spirally arranged material, probably ribonucleoprotein in nature. The length of the spiral is between 2 and 2.5 /k with a pitch of about 16.5 m/k. Further electron microscopic observations confirm previous results and provide new information on the ultrastructural nature of the electron-dense spiral. These are reported in the present communication. The methods of preparation of material have been reported previously (Lecatsas et al., 1969) and consist basically of fixation of virus-infected BHK 21 cells with gluteraldehyde followed by osmium tetroxide, dehydration in ethanol and embedding in Epon 812. Thin sections were then double-stained with uranyl acetate and lead citrate and examined in Siemens Elmiskop 1A electron miscroscope. Close examination of the arrowed portion of the virus particles in Plate 1 (1) reveals that the granular electrondense material represents sectioned strands. It is also evident that the electron-dense material is helical in nature (arrows). Measurement of the individual strands gives an approximate thickness of 4 m/k ( 40 A). An enclosed space between the strands is clearly visible in Plate 1 (3). The total diameter of limb "a" of the virus particle shown in Plate 1 (3) is 18.4 m!J-, and that of
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ورودعنوان ژورنال:
- The Onderstepoort journal of veterinary research
دوره 37 2 شماره
صفحات -
تاریخ انتشار 1970